PID tuning in the air

Hello,

Can I tune the PID and FF while the plane in the air?

I found the result of autotune is always kinda overshooting. what is reason could be? what params should I check before doing autotune? thank you for any help.

There is extensive in-flight tuning:

Read it:
In flight tuning

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Thank you, Dave, for always being helpful and apology for not carefully reading enough.

One thing to confirm. Does it mean from 50 to 57 for fixed wing tune only with one param can be tuned ? a fixed wing cannot have a set of tuning.

Yes, right. The tuning Sets >100 are for Quadplane.