Hover learning problem with different payloads

Can I use different ah batteries 3ah and 52ah on tbs 500 , ver 4.0.1 , suggest me the settings for learning hover

Surely you mean 5.2AH. Hover throttle will be automatically learned by flying in an assisted mode for 1 minute or so.

Assisted mode means ?

Do mean Every time I change my battery I have to wait for 1 min to learn hovering throttle by ardupilot?

Google “arducopter hover learning” and you will find this: